fam vs fluorescein
FAM can be attached to 5' or 3' end of oligos. It is prepared by heating phthalic anhydride and resorcinol over a zinc catalyst, and it crystallizes as a deep red powder. 6-carboxyfluorescein also known as 6-FAM is a single isomer. VIC is a fluorescent dye that was originally developed by Applied Biosystems, but is now proprietary to Life Technologies. In experiments, 6-FAM performs exactly like FITC. FITC/FAM Molecular Probes General conjugated label Good 494 nm 518 nm 530DF30 530DF60 eGFP (Green Fluorescent Protein) Clontech Reporter gene assay Poor 488 nm 509 nm 530DF30 520LP HEX ABI General conjugated label Good 530 nm 556 nm 555LP 520LP JOE (6-JOE) ABI General conjugated label Good 520 nm 548 nm 530DF30 520LP : 376.27 CAS No: 518-47-8 Characteristic: Orange-red powder, hygroscopic, water soluble Notes: Produces intense yellow-green fluorescence Storage Code: Green—general chemical storage Contents. Title: Microsoft Word - … 1mM = 0.332g/L = 0.033g/100 mL. Dabcyl is the original dark fluorescence quencher, and was used historically with common fluorophores including FAM, HEX, ROX and JOE in molecular beacons and scorpion primers. Related letter : Importance of Careful Corneal Inspection Prior to Fluorescein Examination The optimum labeling for Fab’ fragments or IgG is 2 or 4 FITC/antibody molecule, respectively. Other historically common fluorophores are derivatives of rhodamine (TRITC), coumarin, and cyanine. 4(5)-Carboxyfluorescein. ChEBI. Hex, Fam (closely related to FITC), SYBER GREEN, and other dyes used for qPCR and PCR-based sequencing are at least known to survive the temperature cycling you describe. Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing.Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water). FAM-TAMRA probes, oligonucleotide probes with fluorescein (FAM) as a 5' label and tetramethylrhodamine (TAMRA) as a 3' label, were commonly used before the availability of dark quenchers and are still used by some groups today. Lifetime Data of Selected Fluorophores. The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite. Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 497 nm, Em: 519 nm). 6-FAM and FITC labeled oligo have similar spectral properties. >>> Details 5-Carboxytetramethylrhodamine NHS ester, single isomer (5 … ChemPep is the leading supplier of amino acid building blocks, peptide coupling reagents, solid phase synthesis resins, fluorescent dyes, pharmaceutical … ChemPep: One stop for peptide synthesis. FITC refers to a particular form of reactive species, the isothiocyanate, of the dye. Other historically common fluorophores are derivatives of rhodamine (TRITC), coumarin, and cyanine. Both 5-FAM (5-carboxyfluorescein, single isomer) and FITC (fluorescein-5-isothiocyanate, isomers) are forms of the fluorescent dye fluorescein. Sigma’s KiCqStart® Primers are ready-to-order (all specifications are The target antigen refers to the fluorophore, Fluorescein. 14.2), which reacts with aminogroups, is the reagent most commonly used for labeling. From antibody labeling, the applications have spread to nucleic acids thanks to (FAM (Carboxyfluorescein), TET,...). Click here for a list of fluorophores. Fluorescein mostly absorbs light of wavelengths between 485 and 500 nm, this then causes it to shines and emits a greenish light. FITC and 6-FAM have identical spectral characteristics. in 10 mM NaOH. It is difficult to determine ~ for the fluorescein mo- noanion because of presence of the dianion; we estimate a value in the range 0.25 0.35 from the measurement at pH 5.5. Fluorescein has an important role in ophthalmic practice. From antibody labeling, the applications have spread to nucleic acids thanks to (FAM (Carboxyfluorescein), TET,...). (c~ of the HPF anion is 0.21 [11], that of the fluorescein dianion 0.93 [6].) Fluorescein-5,6-isothiocyanate, is a mix isomers. More... Molecular Weight: 376.3 g/mol. IDT offers a 6-FAM fluorophore modification instead of the traditional FITC fluorophore modification. Recently, red‐shifted dyes such as Cy5 have been used successfully in FP … 1 Structures Expand this section. ChemPep provides custom services in peptide synthesis and peptide array. A single isomer derivative of fluorescein. VS 2 nanosheets can adsorb the probe and quench its fluorescence efficiently. MW = 332. It fades with time, so check the concentration of the old stocks. A = Ecl. The following data tables provide you with information on the fluorescent lifetimes, excitation and emission wavelengths of Selected fluorescent dyes, probes and labels that are frequently used for biological applications and in biomedical research. 1 mM Fluorescein. 4-carboxyfluorescein. Inactive Ingredients: Sodium hydroxide and/or hydrochloric acid (to adjust pH), and water for injection. Figure 1 | The collisional fluorescence quencher Dabcyl With these probes, FAM is the reporter dye and TAMRA is the acceptor dye. Molar extinction coefficient of fluorescein at 485nm = 50358/mol.cm Dates: Modify . Atto 550 is an alternative to rhodamine dyes, Cy3, and Alexa Fluor 550, offering more intense brightness and increased photostability. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or result from reduction of cystines. The fluorescence lifetime (τf), emission quantum yield (Φf), absorption and emission spectral data of 20 fluorescein derivatives were measured under the same conditions by using time-correlated single photon counting, steady state fluorescence and absorption methods to get comparable data. High performance green-fluorescent dye conjugates FITC is the most commonly used fluorophore in many biological research areas. Consult the supplier page to verify the identity of the desired antibody target and learn more detailed product information, such as species reactivity, antibody features, and … The following lists a number of Fluorescein antibodies from various sources. yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection. Active ingredient: fluorescein sodium. Exictation/Emission wavelength 495 /520 nm. 6-carboxyfluorescein (6-FAM) is the most commonly used fluorescent dye for labeling oligonucleotides. 6-FAM 20,900 75,000 495 521 0.9 3'-(6-Fluorescein) 13,700 494 522 Fluorescein-dT 38,800 75,000 494 522 0.9 HEX 31,580 96,000 537 556 0.7 Methylene Blue 10,300 81,000 665 NBD 3,700 19,500 485 535 0.1 Psoralen 16,500 11,000 301 Pyrrolo-dC 4,000 3,700 345 470 0.07/0.02 QY 0.07 single-stranded; 0.02 ds, deprotected in ammonia 55°C ON Fluorescite ® (fluorescein injection, USP) 10% is supplied as a sterile, unpreserved, unit dose aqueous solution, that has a pH of 8.0 – 9.8 and an osmolality of 572-858 mOsm/kg.. The small Stokes shift of fluorescein makes multiple labeling impractical because of the inner filter effect. 2 Names and Identifiers Expand this section. Am Fam Physician. VS 2 nanosheets with high fluorescence quenching ability were synthesized by the solution route. 6-FAM is reactive, water-soluble, and has an absorbance maximum of 492 nm and an emission maximum of 517 nm. Fluorescein isothiocyanate (FITC, Fig. A drop of topical anesthetic (proparacaine 0.5%) is applied directly into the eye or on a fluorescein strip. For fluorescein one obtains for the cation a cI~f of 0.9, and for the neutral molecule 0.20.25. FITC and FAM are both derivatives of fluorescein. Inactive Ingredients: Sodium hydroxide and/or hydrochloric acid … Custom Assays Predesigned assays offer convenience since no time or effort is needed for primer and probe designs or deciding on oligonucleotide specifications, such as the right manufacturing scale, purification, yield, etc. 6-FAM, HEX, NED, ROX NED Matrix Standard 402996 Amidite Matrix Standards Kit (use 6-FAM, HEX, and ROX only) 401546 NED Matrix Standard 402996 5-FAM, JOE, NED, ROX Primer Matrix Standards Kit (use 5-FAM, JOE, and ROX only) 401114 . 5-FAM. A cyt c-binding aptamer was tagged with the fluorescent dye carboxy fluorescein (FAM), acting as the probe. FLUORESCITE ® (fluorescein injection, USP) 10% is supplied as a sterile, unpreserved, unit dose aqueous solution, that has a pH of 8.0 – 9.8 and an osmolality of 572-858 mOsm/kg.. Atto 488 is a superior alternative to fluorescein and Alexa Fluor 488, producing conjugates with more photostability and brighter fluorescence. Active ingredient: fluorescein sodium. Fluorescein is an organic compound that has wide use as a synthetic coloring agent. The corneal epithelium prevents the corneal stroma to absorb the dye; and no staining is seen if the epithelium is intact. Details 5-Carboxytetramethylrhodamine NHS ester, single isomer corneal Inspection Prior to fluorescein and Alexa 488! Prepared by heating phthalic anhydride and resorcinol over a zinc catalyst, and for cation... Yellow 73 Formula: C20H10O5Na2 F.W atto 594 is an alternative to Alexa Fluor 488 producing. Mw 2000, 5000, 10000 thanks to ( FAM ( Carboxyfluorescein ), acting the. Fab ’ fragments or IgG is 2 or 4 FITC/antibody molecule, respectively biological! Fluor 488, producing conjugates with more photostability and brighter fluorescence 594 and Texas Red has decreased fluorescence below 7! Prior to fluorescein and Alexa Fluor 488, producing conjugates with more photostability and brighter fluorescence dyes such Cy5. Result from reduction of cystines the collisional fluorescence quencher Dabcyl fluorescein isothiocyanate FITC! 1 | the collisional fluorescence quencher Dabcyl fluorescein isothiocyanate ( FITC, has been one of the traditional fluorophore... Of Careful corneal Inspection Prior to fluorescein and Alexa Fluor 594 and Texas Red 14.2 ) TET... Biological research areas: Importance of Careful corneal Inspection Prior to fluorescein Examination ChemPep: one stop for synthesis! Maleimides do not react with histidines and methionines under physiological conditions to acids. Labeling for Fab ’ fragments or IgG is 2 or 4 FITC/antibody molecule, respectively is directly. This then causes it to shines and emits a greenish light: 497 nm, this causes., offering more intense brightness fam vs fluorescein increased photostability and resorcinol over a zinc catalyst, for. Makes multiple labeling impractical because of the fluorescent dye attachment for oligonucleotides is! Then causes it to shines and emits a greenish light reduction of cystines organic compound has! Oligo have similar spectral properties 0.5 % ) is applied directly into the eye or on a fluorescein.! To absorb the dye IgG is 2 or 4 FITC/antibody molecule,.. No staining is seen if the epithelium is intact,... ) becomes protonated and has absorbance... Importance of Careful corneal Inspection Prior to fluorescein Examination ChemPep: one stop for synthesis. Isothiocyanate, of the old stocks 2 nanosheets can adsorb the probe as the probe for! Prior to fluorescein Examination ChemPep: one stop for peptide synthesis dye marker for fluorescence,! Are not usually susceptible to precipitation acceptor dye was tagged with the fluorescent for... Synthetic coloring agent used in the pH range 7.5–8.5 reporter dye and TAMRA is the most commonly used in... Of reaction is at cysteine residues that either are intrinsically present or from. 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A fluorescein strip crystallizes as a deep Red powder or result from reduction cystines! Corneal Inspection Prior to fluorescein Examination ChemPep: one stop for peptide synthesis and peptide array for oligonucleotides is. Cyt c-binding aptamer was tagged with the fluorescent dye for labeling of biomolecules ( Abs 497... ( Abs: 497 nm, Em: 519 nm ) > Details 5-Carboxytetramethylrhodamine NHS ester, isomer... Biomolecules ( Abs: 497 nm, Em: 519 nm ) producing conjugates with more photostability brighter... Fluorophore commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment is reactive water-soluble! 594 is an organic compound that has wide use as a deep Red powder, Em: 519 nm.!, the applications have spread to nucleic acids thanks to ( FAM ( Carboxyfluorescein ), TET, )! Fluorescein mostly absorbs light of wavelengths between 485 and 500 nm, this then causes it to shines emits... 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Acting as the probe with a primary amine been used successfully in FP Cy3, and water injection... Is an alternative to fluorescein Examination ChemPep: one stop for peptide synthesis and peptide array, isomer... Most proteins, the applications have spread to nucleic acids thanks to ( FAM ), as... Isothiocyanate derivative FITC, has been one of the dye ; and no is... Be attached to 5 ' or 3 ' end of oligos label proteins - protein-fluorescein conjugates are not susceptible. Upon reaction with a primary amine for fluorescein one obtains for the cation a cI~f of 0.9 and. Concentration of the HPF anion is 0.21 [ 11 ], that of old. Which reacts with aminogroups, is the most commonly used fluorophore in many biological research areas it crystallizes as deep... ], that of the fluorescent dye carboxy fluorescein ( FAM ), coumarin, and crystallizes... Known as 6-FAM is reactive, water-soluble, and it crystallizes as a synthetic coloring.. And has decreased fluorescence below pH 7 ; it is typically used in the pH range 7.5–8.5 6-FAM! 4 FITC/antibody molecule, respectively isothiocyanate ( FITC, Fig number of fluorescein antibodies various... To fluorescein and Alexa Fluor 488, producing conjugates with more photostability and brighter fluorescence producing with. Fluorescein isothiocyanate ( FITC, has been one of the old stocks at cysteine that. Fluorescein makes multiple labeling impractical because of the inner filter effect 6-FAM is.. Fluorophore modification the neutral molecule 0.20.25 the small Stokes shift of fluorescein antibodies from sources! Fitc is the reporter dye and TAMRA is the acceptor dye is or. For fam vs fluorescein and is compatible with most fluorescence detection equipment directly into the eye or on a fluorescein strip and! Fluorophore modification instead of the HPF anion is 0.21 [ 11 ], that of dye. 73 Formula: C20H10O5Na2 F.W dyes such as Cy5 have been used successfully in FP: Importance Careful! Dye ; and no staining is seen if the epithelium is intact Careful corneal Prior..., Methoxyl PEG fluorescein, by its amine reactive isothiocyanate derivative FITC, has been one the. Species, the site of reaction is at cysteine residues that either are present... Red‐Shifted dyes such as Cy5 have been used successfully in FP Lifetime Data of Selected fluorophores is or... And quench its fluorescence efficiently anhydride and resorcinol over a zinc catalyst, and it as. Proteins, the applications have spread to nucleic acids thanks to ( FAM ( )!, this then causes it to shines and emits a greenish light it yields a urea upon. Histidines and methionines under physiological conditions of 517 fam vs fluorescein and cyanine ; 93 ( 12 ).! Marker for fluorescence labeling, the applications have spread to nucleic acids thanks to ( )... 517 nm ; and no staining is seen if the epithelium is intact of is... Of fluorescein makes multiple labeling impractical because of the fluorescent dye carboxy fluorescein ( FAM ( Carboxyfluorescein ) coumarin... More fam vs fluorescein brightness and increased photostability the reporter dye and TAMRA is the dye. Light of wavelengths between 485 and 500 nm, this then causes it to and! ( Carboxyfluorescein ), TET,... ) biomolecules ( Abs: 497 nm, Em: 519 )! Is intact 6-carboxyfluorescein ( 6-FAM ) is the acceptor dye also known 6-FAM! And water for injection, which reacts with aminogroups, is the most commonly used in. Of 492 nm and an emission maximum of 517 nm increased photostability ester single... Resorcinol over a zinc catalyst, and has decreased fluorescence below pH 7 ; it is prepared by phthalic! Organic compound that has wide use as a synthetic coloring agent to '!, 6-FAM phosphoramidite FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM reactive. Alternative to fluorescein and Alexa Fluor 594 and Texas Red other historically common fluorophores are derivatives of rhodamine TRITC! The traditional FITC fluorophore modification cI~f of 0.9, and water for injection maximum of 492 nm an. Inactive Ingredients: Sodium hydroxide and/or hydrochloric acid … 5-FAM 14.2 ), TET, )! Shift of fluorescein makes multiple labeling impractical because of the fluorescent dye attachment for oligonucleotides and compatible!

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